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Open call #1

Marker-Assisted Selection (MAS) in strawberry

Marker assisted selection is a method used to select desirable individuals in a breeding programme based on DNA molecular marker patterns in addition to their trait values. This can serve as a valuable tool to assist breeders to select new genotypes more efficiently. In this project, we evaluated the predictive capacity of molecular markers and their usefulness for MAS. The selected markers were published, and have been validated in diverse strawberry germplasm for a reduced number of cases. We focused on identifying molecular markers for the traits listed in the annex at the end of the document, such as disease resistances, fruit quality as well as vegetative and production traits. For the project, a low-density SNP array for Marker Assisted Selection (MAS) were developed in cooperation with an external facility, that performed the genotyping for the breeders selected in this Open Call. The genotyping costs were covered by the BreedingValue consortium.

The objective of this Open Call
The objective of this Open Call #1 was to share this easy-to-use tool with the selected external breeders to facilitate the selection of superior breeding material. In return, the results obtained by the breeders allowed the BreedingValue consortium to validate the low-density SNP array in larger genetic backgrounds.

Selection process
The Open Call #1 closed on 31.12.2021. A total of 3 proposals were received and 3 proposals have been selected for funding. We would like to thank all applicants for their interest and look forward to the collaboration with Angus Softfruit Ltd., Geoplant Vivai and Niwa Berry Breeding!
In this Open Call, we have funded 3 projects à 20,000€.

Further information is available in the Public evaluation report on Open Call #1.

Testimonials from the Open Call Participants

Geoplant Vivai S.r.l. Società agricola

“Geoplant nurseries participated in the Open Call project with 376 different genotypes belonging to defined crossing populations. The approach was based on 96 markers identified in various populations. The provided data allows us to determine if the evaluated selections possess genetic traits useful to consider them as parent lines in the genetic improvement process. The data generated in this open call project will enable us to select high-potential genotypes and identify interesting new crosses. All the information gained from this experience—the genetic heritage of the evaluated selections, relationships between phenotypic traits, potential of both validated and non-validated molecular markers, and general knowledge on marker usage—will represent an important resource for the company's future.”

Michele Montanari, Environmental Engineer

https://www.geoplantvivai.com

Niwa Berry Breeding

“This Open Call project showed us how the genotypic and phenotypic data of the analyzed strawberry genotypes were linked. Combining these data allows for identifying SNP markers that have a large impact on shaping important utility features of strawberries. The results of the Breeding Value Open Call project bring a clear mark of modernity to our breeding programme, making the company more competitive compared to other breeding companies both domestically and internationally. The results will undoubtedly shorten the time required to develop new varieties and bring them to market, which means growth and revenue for the company. We hope that the research will continue, providing knowledge for breeders and greatly facilitating their work. The project work provided an opportunity for our breeders to learn, expand, and acquire new knowledge, which will undoubtedly yield positive results in the progress of our breeding efforts.”

Dr Katarzyna Król-Dyrek, Breeder

https://www.niwabrzezna.pl